Mechanism of the Interaction Between Bromophenol Blue and Bovine Serum Albumin

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Abstract
The mechanism of the interaction between Bromophenol Blue (BPB) and Bovine Serum Albumin (BSA) was studied spectrophotometrically. Absorption spectral changes of BPB–BSA and BPB–Chitosan mixtures in different BSA and Chitosan concentrations are discussed. The influence of different experimental conditions on the absorption spectra of BPB–BSA mixtures was investigated. Through these experiments, the conclusion was drawn that the bathochromic shift of the absorption spectra of the BPB–BSA reaction system results mainly from hydrophobic interaction between BPB and BSA, while electrostatic force is necessary for the formation of the BPB–BSA complex.