Influence of cefoperazone on ethanol and acetaldehyde metabolism in vitro.
- 1 January 1984
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 37 (2) , 167-171
- https://doi.org/10.7164/antibiotics.37.167
Abstract
The effect of cefoperazone on ethanol and acetaldehyde metabolism was studied in rat liver homogenates and with a purified aldehyde dehydrogenase. Rat liver homogenates were incubated with ethanol (30 mM) alone or in combination with cefoperazone (15 or 150 .mu.g/g liver). Ethanol and acetaldehyde concentrations were determined at 6, 12, 18 and 24 min. Cefoperazone added to the incubation medium inhibited ethanol and acetaldehyde metabolism in a concentration-dependent manner. The addition of cefoperazone to rat liver homogenates incubated with acetaldehyde (300 .mu.M) did not inhibit acetaldehyde disappearance for 15 min. Purified aldehyde dehydrogenase was incubated with 300 .mu.M acetaldehyde. When cefoperazone was added, acetaldehyde disappearance was significantly slower than without cefoperazone. Thus, cefoperazone inhibits ethanol metabolism in rat liver homogenates in a concentration-dependent manner. The effect of the antibiotic on acetaldehyde elimination in liver homogenate depends on the concentration of acetaldehyde in the medium. The acetaldehyde dehydrogenase obtained from yeast is inhibited by cefoperazone.This publication has 2 references indexed in Scilit:
- Human Aldehyde Dehydrogenase: Mechanism of Inhibition of DisulfiramScience, 1982
- Disulfiram as a Tool in the Studies on the Metabolism of Acetaldehyde in RatsActa Pharmacologica et Toxicologica, 1976