The Separation of Globin Chains by High Pressure Cation Exchange Chromatography
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 9 (1) , 53-63
- https://doi.org/10.3109/03630268508996982
Abstract
A high performance cation exchange system is described for isolating globin chains. The method is rapid and inexpensive, using total lysate, a 14 min gradient, and a 3 cm column. The running buffers contain 6 M urea to dissociate and unfold the globin, and a NaCl gradient is employed for elution. The procedure provides a convenient way of detecting mutant globins with an intrinsic alteration in their charge.This publication has 10 references indexed in Scilit:
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