Detection of multiallele polymorphisms within gene sequences by GC-clamped denaturing gradient gel electrophoresis.
- 1 March 1992
- journal article
- Vol. 50 (3) , 567-75
Abstract
The ability to efficiently detect DNA polymorphisms is essential for the completion of a high-resolution polymorphic linkage map of the human genome. Currently the most informative polymorphisms are the multiallelic dinucleotide repeat polymorphisms. However, many gene sequences lack an associated dinucleotide repeat sequence. We used GC-clamped denaturing gradient gel electrophoresis to screen for DNA polymorphisms in the following six gene sequences: MCC, p53, prealbumin (transthyretin), rhodopsin, S-antigen, and TGF-alpha. A single-base sequence polymorphism was identified in each of these gene sequences. Some of these polymorphisms were multiallelic and highly informative. Our results demonstrate the value of denaturing gradient gel electrophoresis for both identifying and analyzing human DNA polymorphisms. The ability to detect highly informative polymorphisms within gene sequences will greatly contribute to a gene-based polymorphic linkage map.This publication has 36 references indexed in Scilit:
- The p53 tumour suppressor geneNature, 1991
- DNA sequence polymorphisms in Alu repeatsGenomics, 1990
- Informativeness of human (dC-dA)n · (dG-dT)n polymorphismsGenomics, 1990
- Use of denaturing gradient gel electrophoresis to detect point mutations in the factor VIII geneGenomics, 1990
- Hereditary amyloidosis: detection of variant prealbumin genes by restriction enzyme analysis of amplified genomic DNA sequencesClinical Genetics, 1990
- Identification of the Cystic Fibrosis Gene: Chromosome Walking and JumpingScience, 1989
- Identification of the Cystic Fibrosis Gene: Genetic AnalysisScience, 1989
- Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNAScience, 1989
- The sequence of human retinal S‐antigen reveals similarities with α‐transducinFEBS Letters, 1988
- Hypervariable ‘minisatellite’ regions in human DNANature, 1985