The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (24) , 10373-10391
- https://doi.org/10.1093/nar/15.24.10373
Abstract
We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb RNA and a minor 3.9-kb RNA. The 1.7-kb species appears to be the functional mRNA; the role of the 3.9-kb RNA, which extends further in the 5′ direction, is unclear. The actual size of the hU1-70K protein is probably 52 kd, rather than 70 kd. The protein contains three regions similar to known nucleic acid-binding proteins, and it binds RNA in an in vitro assay. Comparison of the cDNA sequences indicates that there are multiple subclasses of mRNA that arise by alternative pre-mRNA splicing of at least four alternative exon segments. This suggests that multiple forms of the hU1-70K protein may exist, possibly with different functions in vivo.Keywords
This publication has 32 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Molecular cloning of cDNA for CENP-B, the major human centromere autoantigenThe Journal of cell biology, 1987
- The role of small nuclear ribonucleoprotein particles in pre-mRNA splicingNature, 1987
- PRE-mRNA SPLICINGAnnual Review of Genetics, 1986
- A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoproteinCell, 1986
- Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of YeastCell, 1986
- A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: Domain structure and expressionCell, 1986
- Small nuclear ribonucleoprotein particle assembly in vivo: Demonstration of a 6S RNA-free core precursor and posttranslational modificationCell, 1985
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- α-Helix–double helix interaction shown in the structure of a protamine-transfer RNA complex and a nucleoprotamine modelNature, 1978