GROWTH-INHIBITION AND DIFFERENTIATION OF HUMAN SALIVARY ADENOCARCINOMA CELLS BY MEDIUM CONDITIONED WITH NORMAL HUMAN-FIBROBLASTS

  • 15 May 1988
    • journal article
    • research article
    • Vol. 48  (10) , 2819-2824
Abstract
The present study demonstrates that normal human fibroblasts (WI-38) exert a profound influence on the growth and differentiation of HSCc-C5a clonal neoplastic epithelial cell line of human salivary gland origin. Coculture of HSCc-C5 with WI-38 resulted in a slowing of growth and an increase in glycosaminoglycan synthesis by an indirect effect involving diffusible factor(s). Conditioned medium (CM) from WI-38 grown in Dulbecco''s modified Eagle''s medium supplemented with 10% fetal bovine serum affected HSGc-C5 as follows. The CM suppressed growth of monolayer cells; inhibited DNA synthesis; suppressed growth (decrease in size of colonies) in semisolid agar; stimulated glycosaminoglycan synthesis; induced expression of functional markers of the salivary gland, such as the secretory component, lactoferrin, and lysozyme; inhibited expression of alkaline phosphatase; and induced morphological alteration mo elongated cells. These findings strongly suggest that WI-38 CM certains a factor(s) which inhibits growth and induces differentiation of H5Gc-C5. The CM was also active on other human cancer cells as a growth inhibitor, but not on normal human fibroblasts. Partial purification and characterization of the factor(s) suggests that it may be a novel protein carrying both tumor inhibiting and differentiation inducing activities.