PROCEDURE FOR DETERMINATION OF ALDOSTERONE IN HUMAN PERIPHERAL PLASMA BY DOUBLE-ISOTOPE DERIVATIVE ASSAY, AND ITS APPLICATION FOR MEASUREMENT OF SECRETORY RATE AND URINARY EXCRETION
- 1 December 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 45 (12) , 1919-1936
- https://doi.org/10.1139/o67-225
Abstract
This is a report of a procedure for the determination of peripheral plasma aldosterone, in which14C-labeled aldosterone serves as the marker, and the acetylating agent is relatively inexpensive tritium-labeled acetic anhydride of high specific activity. A new method for the chromatographic purification of aldosterone and aldosterone diacetate is presented. This procedure provides a better separation of these two compounds from the unlabeled and labeled impurities.This publication has 6 references indexed in Scilit:
- DETERMINATION OF Δ4-3-KETO-CORTICOSTEROIDS IN HUMAN PERIPHERAL PLASMA BY THE ISONICOTINIC ACID HYDRAZIDE (INH) REACTION*Journal of Clinical Endocrinology & Metabolism, 1957
- DETAILED STUDY OF A PURIFIED URINARY ALDOSTERONE FRACTIONCanadian Journal of Biochemistry and Physiology, 1956
- Determination of Organic Compounds as Isotopic Derivatives.1 II. Amino Acids by Paper Chomatographic and Indicator Techniques2Journal of the American Chemical Society, 1950