Imaging diacylglycerol dynamics at organelle membranes
- 21 September 2006
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 3 (10) , 797-799
- https://doi.org/10.1038/nmeth930
Abstract
Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes.Keywords
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