Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA

Open Access

1 January 1982

journal article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 10 (24) , 7947-7957
https://doi.org/10.1093/nar/10.24.7947

Abstract

Summary Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon Cloning in bacteriophage. M13mp9, two recombinant phages were selected to construct a p8R322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously establishment sequence of another PSTV strain, three of which were base exchanges and one a deletion.

Keywords

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