Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
- 20 November 1981
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 214 (4523) , 916-919
- https://doi.org/10.1126/science.6272391
Abstract
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.This publication has 12 references indexed in Scilit:
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