The Effects of an Extract of Blood Cells upon the Cultivation of Bacterium tularense in Liquid Media

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Abstract
A blood cell extract (BCE) was prepd. by heating and acidifying laked blood cells, filtering off coagula, concentrating the filtrate in vacuo, neutralizing, and freeze-drying the concentrate. In basal media made with gelatin hydrolysate, cystine, glycerol, both with and without salts, addition of BCE permits a sustained growth of B. tularense from small inocula. With appropriate equipment the media are suitable for mass cultivation. Since all constituents are dialyzable, large quantities of cells can be obtained in a chemically pure state. In addition to stimulating growth and providing all necessary factors and substrates to the basal media, BCE can be made to effect a metabolic stabilization of the pH of cultures in unbuffered media owing to its property of regulating amine production from amino aoid utilization and acid production from carbohydrate utilization concurrently. These media are suitable for many strains and many purposes.

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