A new ATP-independent DNA endonuclease fromSchizosaccharomyces pombethat recognizes cyclobutane pyrimidine dimers and 6–4 photoproducts
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (15) , 3026-3032
- https://doi.org/10.1093/nar/22.15.3026
Abstract
We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.Keywords
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