The construction and replication properties of hybrid plasmids composed of the r-determinant of R100.1 and the plasmids pCRI or pSC201
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 168 (3) , 337-340
- https://doi.org/10.1007/bf00271505
Abstract
We have cloned the entire r-determinant of the antibiotic resistance plasmid R100.1 on the plasmid vectors pCR1 and pSC201. We find that the hybrid plasmids segregate from cultures in which replication of the vector is blocked. This suggests that the r-det is not capable of autonomous replication.This publication has 23 references indexed in Scilit:
- Properties of an Autonomous r-Determinant from R100.1Cold Spring Harbor Symposia on Quantitative Biology, 1979
- Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1Molecular Genetics and Genomics, 1977
- Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmidMolecular Genetics and Genomics, 1977
- Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genesMolecular Genetics and Genomics, 1977
- Replication control in a composite plasmid constructed by in vitro linkage of two distinct repliconsNature, 1976
- Effects of different alleles of the E. coli K12 polA gene on the replication of non-transferring plasmidsMolecular Genetics and Genomics, 1976
- A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acidMolecular Genetics and Genomics, 1976
- Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: II. Structure of drug resistance (R) factors and f factorsJournal of Molecular Biology, 1973
- Carcinogenic Hydrocarbons and Human Cells in CultureNature, 1971
- Multiple Molecular Species of Circular R-factor DNA isolated from Escherichia collNature, 1969