Abstract
Several aspects of the properties and preparation of human plasmin are considered. It appears that glycerol activation of the proenzyme is a peculiar property dependent in part on the structure of the glycerol molecule since closely related compounds are without effect. Observations on the influence of ionic strength, on ion activation and inhibition of the enzyme, on substrate specificities, and on preparative procedures are recorded and comparisons with similar data on related bovine enzymes are made.

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