RENAL TUBULAR TRANSPORT: ACCUMULATION OF P-AMINOHIPPURATE BY RABBIT KIDNEY SLICES

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Abstract
Thin slices of rabbit kidney cortex, incubated in a saline medium containing dilute p-aminohippurate (PAH), are capable of accumulating PAH against a considerable concn. gradient. PAH accumulation is coupled with respiration and can be strikingly modified by the addition of various metabolic intermediates.. Acetate and certain acetyl precursors stimulate PAH uptake; members of the Krebs cycle, most fatty acids and certain amino acids inhibit. Effects of various enzyme inhibitors were examined. Accumulation by the slice appears to be related to the tubular excretion of PAH by the intact kidney. Acetate is suggested as one of the rate-limiting cellular components of the PAH transport mechanism.