13C-n.m.r. study of citrate metabolism in rabbit renal proximal-tubule cells
- 15 January 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 257 (2) , 425-429
- https://doi.org/10.1042/bj2570425
Abstract
The metabolism of a 13C-labelled substrate, [3-13C]citrate, was monitored in rabbit renal proximal-tubule cells by 13C n.m.r. The relative enrichments of label in glutamate, glutamine and alpha beta-glucose allowed a calculation of the rate of the glutamate dehydrogenase flux relative to the flux via phosphoenolpyruvate carboxykinase. A ratio of 1.2 +/- 0.05 was found. Under steady-state conditions of active gluconeogenesis, the ratio of flux through pyruvate kinase to the gluconeogenic rate was 0.97 +/- 0.03.This publication has 8 references indexed in Scilit:
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