Use of Single Leaf Cells to Study Mode of Action of SAN 6706 on Soybean and Cotton

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Abstract
Enzymatically isolated leaf cells from cotton (Gossypium hirsutumL. ‘Stoneville’) and soybean (Glycine max(L) Merr. ‘Kino’) were used to study the effect of SAN 6706 [4-chloro-5-(dimethylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone] on photosynthesis, protein, RNA, and lipid synthesis during the first 19 hr of herbicidal treatment. Cotton plants are tolerant to SAN 6706, whereas soybean plants are susceptible. SAN 6706 inhibited the incorporation of14C-bi-carbonate, uridine, and acetate into cotton leaf cells but stimulated incorporation of leucine. Incorporation of all of these precursors into soybean cells was inhibited. Cotton and soybean cells each took up equivalent amounts of labeled SAN 6706. The incorporation activity of the cotton and soybean cells appeared to regulate the entrance of the precursors into the cells by depleting the intracellular precursor pools. It is suggested that the inhibition of RNA and lipid synthesis in cotton and soybean cells is an indirect result of inhibited photosynthesis. Isolated cotton cells were not tolerant of the herbicide.