Encapsulation of oligonucleotides in stealth Me.PEG-PLA50nanoparticles by complexation with structured oligopeptides
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Drug Delivery
- Vol. 3 (3) , 187-195
- https://doi.org/10.3109/10717549609029449
Abstract
Two oligopeptides with alternating hydrophilic—hydrophobic amino acids, H-(leu-lys-lys-leu)10-OH and H-(leu-lys-leu-lys)10-OH, were shown to have higher affinity for a 13-mer oligonucleotide than H-(pro-lys-lys-leu)10-OH used as a control. This increased affinity was correlated to the secondary structure adopted by the oligopeptides (respectively, α-helix and β-sheet for LKKL and LK) when complexed to the oligonucleotide. Tight ion-pairing association between the phosphate groups of the oligonucleotide and the lysines of the oligopeptide led to efficient encapsulation of the resulting oligonucleotide/oligopeptide non-water-soluble complex in hydrophobic Me.PEG-PLA50 nanoparticles, by coprecipitation with the co-polymer.Keywords
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