Ethylene Production by Auxin-Deprived, Suspension-Cultured Pear Fruit Cells in Response to Auxins, Stress, or Precursor
Open Access
- 1 December 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 73 (4) , 1013-1019
- https://doi.org/10.1104/pp.73.4.1013
Abstract
Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 106 cells per hour) of ethylene in response to auxins, CuCl2 or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achieved about 12 hours after the addition of optimal amounts of indoleacetic acid (IAA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 4 to 5 hours after the addition of CuCl2 and 1 to 2 hours after the addition of ACC. Supraoptimal concentrations of IAA result in a lag phase followed by a normal response. High concentrations of NAA and 2,4-D result in an early (4-5 hours) stress response and injury.Keywords
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