5-methylcytosine is localized in nucleosomes that contain histone H1.
- 1 September 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (18) , 5490-5494
- https://doi.org/10.1073/pnas.80.18.5490
Abstract
A procedure was developed for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated nucleosomes of mouse cell chromatin. At least 80% of the 5-methylcytosine is localized in nucleosomes that contain histone H1. Native nucleosomes that lack histone H1 or possess high mobility group proteins package DNA that is 1.6-2.3-fold undermethylated. The preferential association of methylated sequences with histone H1 has functional significance because DNA methylation has been linked to gene inactivation and histone H1 is known to promote chromatin condensation.This publication has 38 references indexed in Scilit:
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