A simple and efficient method for studying neurotransmitter releasein vitroby a radiotracer technique

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Abstract
A method which allows measurement of neurotransmitter release from isolated preparations has been developed. With this method release of 3H‐acetylcholine from guinea pig ileum and rabbit jejunum could be studied after preincubation of the preparations with 3H‐choline. The release of noradrenaline from guinea pig vas deferens could also be measured after preincubation with 3H‐noradrenaline. The method is based on a device which allows experiments on 4 preparations at the same time, including incubation, field stimulation and collection of fractions for counting of radioactivity. With the purpose of obtaining a simple and inexpensive device for stimulating the tissue preparations electrically, a physiological stimulator distributor (PSD) was constructed. It is concluded that the described method constitutes an inexpensive simple and quick means of studying neurotransmitter release from isolated tissues.

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