Introduction of a CuA site into the blue copper protein amicyanin from Thiobacillus versutus
- 22 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 365 (1) , 92-94
- https://doi.org/10.1016/0014-5793(95)00429-d
Abstract
The C‐terminal loop of the blue copper protein amicyanin, which contains three of the four active site ligands, has been replaced with a CuA binding loop. The purple protein produced has visible and EPR spectra identical to those of a CuA centre. Recent evidence strongly suggests that the CuA centre of cytochrome c oxidase and the A centre of nitrous oxide reductase are similar and are both binuclear. It therefore follows that the purple amicyanin mutant created here also possesses a binuclear CuA centre.Keywords
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