A RADIOIMMUNOASSAY FOR GROWTH HORMONE RELEASE‐INHIBITING HORMONE: METHOD AND QUANTITATIVE TISSUE DISTRIBUTION

Abstract
A specific antiserum has been produced to haemocyanin conjugated synthetic growth hormone release inhibiting hormone (GHRIH). This has allowed the development of a radioimmunoassay for GHRIH sensitive to 5 pg/tube. GHRIH content in 2 m acetic acid extracts of rat tissues have been measured and show the majority to be in CNS—especially hypothalamus and septum and preoptic areas with substantial amounts in spinal cord and thalamus. Extra neurological localization in pancreas, gastric antrum, colon and thyroid have also been demonstrated.