Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway.
- 30 April 1996
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (9) , 4421-4424
- https://doi.org/10.1073/pnas.93.9.4421
Abstract
The Rev protein of HIV-1 is essential for the nuclear export of incompletely spliced viral mRNAs. This action depends on the mutationally defined Rev activation domain, which both binds the nucleoporin-like human cellular cofactor Rab/hRIP and also functions as a nuclear export signal. Protein kinase inhibitor alpha (PKI) also contains a potent nuclear export signal. However, PKI plays no role in nuclear RNA export and instead induces the nuclear export of a specific protein target, the catalytic subunit of cAMP-dependent protein kinase. Here, it is demonstrated that the nuclear export signal of PKI not only binds the Rab/hRIP cofactor specifically but also can effectively substitute for the Rev activation domain in mediating the nuclear export of HIV-1 mRNAs. We conclude that HIV-1 Rev and PKI act through an identical nuclear export pathway and that Rev, rather than using a dedicated RNA export pathway, is instead acting as an adaptor that allows viral mRNAs to access a cellular protein export pathway.Keywords
This publication has 26 references indexed in Scilit:
- A human nucleoporin-like protein that specifically interacts with HIV RevNature, 1995
- Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeastCell, 1995
- Sequence-specific RNA binding by the HIV-1 Rev proteinNature, 1989
- Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitroNature, 1989
- Functional dissection of the HIV-1 Rev trans-activator—Derivation of a trans-dominant repressor of Rev functionCell, 1989
- A novel genetic system to detect protein–protein interactionsNature, 1989
- The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNANature, 1989
- rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.Proceedings of the National Academy of Sciences, 1989
- HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNACell, 1986
- A second post-transcriptional trans-activator gene required for HTLV-III replicationNature, 1986