Characterization of the Exposed Carbohydrates on the Sheath Surface of In vitro-Derived Brugia pahangi Microfilariae by Analysis of Lectin Binding

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Abstract
Adult female B. pahangi [from the abdominal cavity of Meriones unguiculatus] were maintained metabolically active in vitro for up to 35 days in Click''s medium supplemented with 10% horse serum. For the first 14-18 days microfilariae were released into culture. Although these in vitro-derived microfilariae were morphologically identical to in vivo-derived microfilariae, they could be differentiated by their characteristic of binding to a panel of fluorescein-conjugated lectins. Apparently, maturation and release of microfilariae are correlated with glycosidic alterations on the sheath surface.