Isolation of tocopherol‐binding proteins from the cytosol of smooth muscle A7r5 cells
Open Access
- 1 October 1992
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 209 (1) , 37-42
- https://doi.org/10.1111/j.1432-1033.1992.tb17258.x
Abstract
Cultured smooth muscle A7r5 cells were able to take up α-tocopherol (32 ± 1.2 nmol/mg protein) the largest part of which (60%) was present in the cytosolic fraction. Using a tocopherol-based affinity chromatography and α-, β-, γ-, and δ-tocopherols as eluants, three polypeptides of molecular masses 81, 58 and 31 kDa were eluted. This preparation had α-[3H]tocopherol binding capability. The 58-kDa polypeptide could also be eluted by chromanol and the 81-kDa polypeptide could be eluted also by phytol. The 81-kDa polypeptide had the unique P-E-E-d-Q-X-Q-Y N-terminal sequence.Keywords
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