Activation of Anthranilic Acid andN-Methylanthranilic Acid by Cell-free Extracts fromRuta graveolensTissue Cultures

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Abstract
Cell-free extracts of Ruta graveolens tissue cultures obtained after (NH4)2SO4 precipitation and gel filtration on Sephadex G-25 catalyze the ATP-dependent activation of N-methylanthranilic acid according to the hydroxamate method. The rate of the enzyme reaction was shown to be linear for about 75 min and was proportional to the protein concentration up to at least 0.75 mg/ml of the enzyme preparation. This particular enzyme activity was only detectable in acridone alkaloid producing tissue cultures. Apparently activation of N-methylanthranilic acid is the second pathway-specific step in acridone alkaloid biosynthesis. It is not yet clarified if the activation takes place via the corresponding acyl adenylate or via phosphorylation.