On RNA-polymerases of leukemia L1210 origin and an enzymatic method to screen antitumor antibiotics.
- 1 January 1977
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 30 (6) , 500-505
- https://doi.org/10.7164/antibiotics.30.500
Abstract
Four DNA dependent RNA polymerases were separated from homogenate of mouse Leukemia L1210 cells by DEAE cellulose column chromatography and tentatively designated as peaks I, II, III and IV in elution order. Peak II was inactivated by the addition of .alpha.-amanitin and effects of antibiotics and enzymes on the RNA polymerase activity using peaks I, II and a mixture of peaks I and II were examined. The RNA polymerases were used to screen for enzyme inhibitors produced by microbes. This enzymatic method was successfully proved to select antitumor antibiotics.This publication has 4 references indexed in Scilit:
- Studies on the pathogenesis of liver necrosis by α-amanitin. Effect of α-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nucleiBiochemical Journal, 1967
- Studies on the stimulation by ammonium sulphate of the DNA-dependent RNA polymerase of isolated rat-liver nucleiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- SARCOMYCIN INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN EHRILICH ASCITES CARCINOMA CELLS1963
- Thermal Conversion of Nonpriming Deoxyribonucleic Acid to PrimerJournal of Biological Chemistry, 1959