A subcloning strategy for DNA sequence analysis
Open Access
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (23) , 5541-5549
- https://doi.org/10.1093/nar/8.23.5541
Abstract
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are generated. These can be sequenced directly without further strand- or fragment separation steps.Keywords
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