PRODUCTION OF COLONY-STIMULATING FACTOR(S) FOR GRANULOCYTE-MACROPHAGE AND MULTIPOTENTIAL (GRANULOCYTE ERYTHROID MEGAKARYOCYTE MACROPHAGE) HEMATOPOIETIC PROGENITOR CELLS (CFU-GEMM) BY CLONAL LINES OF HUMAN IL-2-DEPENDENT LYMPHOCYTES-T

  • 1 January 1984
    • journal article
    • research article
    • Vol. 12  (9) , 720-727
Abstract
Human T-lymphocyte lines that were selected for recognition of HLA-DR6 antigen and were dependent for growth in vitro on an added source of interleukin-2 (IL-2) were derived from the peripheral blood of normal individuals. Each was tested for production of a lymphokine(s) with properties of granulocyte-macrophage colony-stimulating factor (GM-CSF) using as target cells nonadherent cells from human long-term bone marrow cultures (LTBMC) or fresh marrow. Each of 8 T-lymphocyte lines that were OKT3, OKT4 and HLA-DR positive produced GM-CSF that stimulated colony formation by both LTBMC cells and fresh marrow. Individually examined single-cell-derived bone marrow colonies growing in T-cell GM-CSF contained peroxidase-positive neutrophils, and macrophage-monocytes (GM-CFUc). Supernatant from a single-cell-derived T-cell clonal line designated F1 stimulated formation of granulocyte-macrophage colonies, megakaryocyte colonies, macroscopic erythroid bursts, and multipotential colonies containing erythroid cells, megakaryocytes, neutrophilic and eosinophilic granulocytes, and monocyte-macrophages (CFU-GEMM) in the presence of added erythropoietin. Human IL-2-responsive T-lymphocytes apparently produce lymphokine(s) that stimulate proliferation of primitive as well as committed hematopoietic stem cells, and implicate human T-lymphocytes in regulation of human multipotential hematopoietic stem cells in vivo.