[3H]Muscimol Binding Site on Purified Benzodiazepine Receptor
- 1 April 1983
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 41 (4) , 1183-1185
- https://doi.org/10.1111/j.1471-4159.1983.tb09070.x
Abstract
The presence of a [3H]muscimol binding site on the purified benzodiazepine receptor was demonstrated. The purified protein was apparently homogeneous as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis (stained with silver), with a molecular weight of 60,000 ± 3000. The benzodiazepine binding sites were characterized as being of the central type and the [3H]flunitrazepam binding was enhanced by GABA. This activation was antagonized by bicuculline. [3H]Muscimol specifically binds to the benzodiazepine receptor. The Scatchard plot indicates a Kd of 23 nM and the ratio [3H]flunitrazepam/[3H]muscimol is approximately unity.Keywords
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