Ion-exchange Chromatography of Oxytocin, Arginine-vasopressin, and Lysine-vasopressin.
- 1 February 1954
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 85 (2) , 226-228
- https://doi.org/10.3181/00379727-85-20838
Abstract
Oxytocin, lysine-vasopressin, and arginine-vasopressin can be chromatographed on ion exchange resin IRC-50 (XE-64). Complete separation of the 3 hormones was achieved by passage through a column buffered at pH 6.95 with 0.2 [image] phosphate buffer; under these conditions lysine-vasopressin and arginine-vasopressin emerged at 40 and 70 effluent ml., respectively, while oxytocin passed through without retardation. At pH 6.00, with 0.2 [image] phosphate buffer as the eluting agent, oxytocin, synthetic oxytocin, and a mixture of natural and synthetic oxytocin chromatographed as a single substance.Keywords
This publication has 14 references indexed in Scilit:
- ELECTROPHORETIC STUDIES OF OXYTOCIN AND VASOPRESSINJournal of Biological Chemistry, 1953
- ELECTROPHORETIC PROPERTIES OF OXYTOCINJournal of Biological Chemistry, 1953
- A CHROMATOGRAPHIC INVESTIGATION OF PANCREATIC RIBONUCLEASEJournal of Biological Chemistry, 1953
- CHROMATOGRAPHIC STUDIES ON LYSOZYMEJournal of Biological Chemistry, 1953
- Further Distribution Studies on the Oxytocic Hormone of the Posterior Lobe of the Pituitary Gland and the Preparation of an Active Crystalline FlavianateJournal of Biological Chemistry, 1952
- Oxytocic Activity of Purified VasopressinExperimental Biology and Medicine, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- THE PURIFICATION AND THE AMINO ACID CONTENT OF VASOPRESSIN PREPARATIONSJournal of Biological Chemistry, 1951
- Preparation of Cytochrome c with the Aid of Ion Exchange Resin.Acta Chemica Scandinavica, 1950
- PREPARATION OF HIGH POTENCY OXYTOCIC MATERIAL BY THE USE OF COUNTER-CURRENT DISTRIBUTIONJournal of Biological Chemistry, 1949