myo-Inositol Trisphosphate Mobilizes Calcium from Fusogenic Carrot (Daucus carota L.) Protoplasts
- 1 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (2) , 395-398
- https://doi.org/10.1104/pp.83.2.395
Abstract
To determine whether or not inositol trisphosphate (IP3) mobilizes calcium in higher plant cells, we investigated the effect of IP3 on Ca2+ fluxes in fusogenic carrot (Daucus carota L.) protoplasts. The protoplasts were incubated in 45Ca2+-containing medium and the 45Ca2+ associated with the protoplasts was monitored with time. Addition of IP3 (20 micromolar) caused a 17% net loss of the accumulated 45Ca2+ within 4 minutes. There was a reuptake of 45Ca2+ and the protoplasts recovered to their initial value by 10 minutes. Phytic acid (IP6), also stimulated 45Ca2+ efflux from the protoplasts. Both the IP3- and the IP6-induced 45Ca2+ efflux were inhibited by the calmodulin antagonist, trifluoperazine.This publication has 14 references indexed in Scilit:
- Inositol phosphates: Profusion and confusionNature, 1986
- Polyphosphoinositides are present in plant tissue culture cellsBiochemical and Biophysical Research Communications, 1985
- Intracellular Calcium and Calmodulin Involvement in Protoplast FusionPlant Physiology, 1985
- Release of Ca2+ from plant hypocotyl microsomes by inositol-1,4,5-trisphosphateBiochemical and Biophysical Research Communications, 1985
- Release of Ca2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphateBiochemical Journal, 1984
- Turnover of Inositol Phospholipids and Signal TransductionScience, 1984
- Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphateNature, 1983
- Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositolBiochemical Journal, 1983
- Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormonesBiochemical Journal, 1983
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951