Cultivation of Lansing Poliomyelitis Virus in Tissue Culture. II. Utilization of Glucose in Synthetic Medium.

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Abstract
Propagation of the Lansing strain of poliomyelitis has been demonstrated in cultures of human embryonic brain and cord. The source of nutriment was a synthetic medium, mixture 199, devised by Morgan, Morton and Parker. A comparison of this medium with Hanks-Simms medium indicated a longer period of cell survival when the former medium was used. Studies on glucose utilization of control and infected tissue cultures indicated a lower level of glucose metabolism in cultures containing the poliomyelitis virus than in uninfected controls. Glucose utilization studies may be a more sensitive indication of the metabolic state of the cell than visual detns. of pH values of cultures.