Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa.
- 1 July 2000
- journal article
- Vol. 14 (13) , 1589-94
Abstract
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polkappa), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polkappa bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polkappa in the mutagenic bypass of certain types of DNA lesions.Keywords
This publication has 30 references indexed in Scilit:
- Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein DinBGenes to Cells, 1999
- hRAD30 Mutations in the Variant Form of Xeroderma PigmentosumScience, 1999
- Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, PolηScience, 1999
- The high spontaneous mutation rate: Is it a health risk?Proceedings of the National Academy of Sciences, 1997
- Translesional Synthesis on DNA Templates Containing a Single Abasic SiteJournal of Biological Chemistry, 1997
- Abasic Translesion Synthesis by DNA Polymerase β Violates the “A-rule”Journal of Biological Chemistry, 1997
- Deoxycytidyl transferase activity of yeast REV1 proteinNature, 1996
- Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζScience, 1996
- dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologuesMutation Research Letters, 1995
- Instability and decay of the primary structure of DNANature, 1993