Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ
- 14 June 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 272 (5268) , 1646-1649
- https://doi.org/10.1126/science.272.5268.1646
Abstract
The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of ∼10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase α was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase ζ.Keywords
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