Antigen-binding capacity and affinity of ferritin conjugated antibodies.

  • 1 October 1973
    • journal article
    • Vol. 25  (4) , 687-95
Abstract
Anti-DNP-globulin from hyperimmunized rabbits was conjugated to ferritin by means of the bifunctional reagent, metaxylene diisocyanate. About 37 per cent of the antibody linked to ferritin was still able to combine specifically with the antigen, whereas no precipitating activity could be demonstrated. Data obtained in equilibrium dialysis experiments indicate an unchanged valency and affinity of those immunoferritin conjugates which remained active.