Evidence for involvement of A‐kinase anchoring protein in activation of rat arterial KATP channels by protein kinase A
Open Access
- 1 October 2001
- journal article
- Published by Wiley in The Journal of Physiology
- Vol. 536 (2) , 421-427
- https://doi.org/10.1111/j.1469-7793.2001.0421c.xd
Abstract
1 We have investigated the possible role of A-kinase anchoring proteins (AKAPs) in protein kinase A (PKA) signalling to ATP-sensitive K+ (KATP) channels of rat isolated mesenteric arterial smooth muscle cells using whole-cell patch clamp and peptides that inhibit PKA-AKAP binding. 2 Intracellular Ht31 peptide (20 μm), which inhibits the PKA-AKAP interaction, blocked KATP current activation by either dibutyryl cAMP or calcitonin gene-related peptide. Ht31-proline (20 μm), which does not inhibit PKA binding to AKAP, did not block KATP current activation. 3 Ht31 reduced KATP current activated by pinacidil and also prevented its inhibition by Rp-cAMPS, effects consistent with Ht31 blocking steady-state KATP channel activation by PKA. However, Ht31 did not prevent KATP current activation by the catalytic subunit of PKA. 4 An antibody to the RII subunit of PKA showed localization of PKA near to the cell membrane. Our results provide evidence that both steady-state and receptor-driven activation of KATP channels by PKA involve the localization of PKA by an AKAP.Keywords
This publication has 29 references indexed in Scilit:
- Targeting of an A Kinase-anchoring Protein, AKAP79, to an Inwardly Rectifying Potassium Channel, Kir2.1Published by Elsevier ,2001
- Angiotensin II inhibits rat arterial KATP channels by inhibiting steady‐state protein kinase A activity and activating protein kinase CeThe Journal of Physiology, 2001
- Regulation of NMDA Receptors by an Associated Phosphatase-Kinase Signaling ComplexScience, 1999
- ATP‐sensitive K+ channel activation by calcitonin gene‐related peptide and protein kinase A in pig coronary arterial smooth muscleThe Journal of Physiology, 1998
- Potassium channels in the vasculatureCurrent Opinion in Nephrology and Hypertension, 1998
- Protein Kinase A-anchoring Inhibitor Peptides Arrest Mammalian Sperm MotilityJournal of Biological Chemistry, 1997
- Mutational Analysis of the A-Kinase Anchoring Protein (AKAP)-binding Site on RIIPublished by Elsevier ,1996
- Vasoconstrictors inhibit ATP-sensitive K+ channels in arterial smooth muscle through protein kinase C.The Journal of general physiology, 1996
- Coordination of Three Signaling Enzymes by AKAP79, a Mammalian Scaffold ProteinScience, 1996
- Anchoring of protein kinase A is required for modulation of AMPA/kainate receptors on hippocampal neuronsNature, 1994