Isolation and identification of a μ‐calpain‐protein kinase Cα complex in skeletal muscle
- 6 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (1) , 60-64
- https://doi.org/10.1016/0014-5793(95)00014-z
Abstract
A μ-calpain-PKC complex was isolated from rabbit skeletal muscle by ultracentrifugation and by anion-exchange chromatography. The PKC associated to μ-calpain was stimulated by calcium, phosphatidylserine and diacylglycerol, and corresponds to a conventional PKC (cPKC). This complex presents an apparent molecular mass close to 190 kDa and is composed of one μ-calpain molecule and of one cPKC molecule. Using monoclonal antibodies specific for the different cPKC isoforms, the isoenzyme associated to μ-calpain was identified as cPKCα. Immunofluorescence staining reveals a co-localization of μ-calpain and cPKCα on the muscle fibre plasma membranesKeywords
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