Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus)

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Abstract
An electrophoretic analysis of radioactively labeled, purified, empty and DNA-containing infectious bovine rhinotracheitis virions revealed the presence of 25-33 structural (virion) polypeptides. A total of 11 of these polypeptides could be labeled with [3H]glucosamine and were identified as glycoproteins. In addition to the 25 structural polypeptides, infectious bovine rhinotracheitis virus-infected [Madin-Darby bovine kidney or Georgia bovine kidney] cells also contained at least 15 nonstructural (nonvirion) polypeptides that were not present in purified virions. Expression of the viral polypeptides in infected cells was controlled temporally. Most viral polypeptides could be categorized as .alpha. (immediate early), .beta. (early) or .gamma. (late) on the basis of their order of appearance in infected cells and whether their syntheses were dependent upon prior viral protein or DNA synthesis. None of the glycoproteins belonged to the .alpha. class, although at least 1 (GVP11) was synthesized in the absence of viral DNA synthesis. Serum from a cow in which infectious bovine rhinotracheitis virus lesions were reactivated by dexamethasone precipitated structural and nonstructural polypeptides.