Rapid steady‐state analysis of blood‐brain glucose transfer in rat
- 1 April 1980
- journal article
- research article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 108 (4) , 331-339
- https://doi.org/10.1111/j.1748-1716.1980.tb06541.x
Abstract
A new kinetic analysis of blood-brain glucose transport is described, based on a steady-state model that takes account of cerebral blood flow, mean capillary glucose concentration and cerebral metabolic rate. The maximal rate (Tmax) and half-saturation constant (Km) of glucose transport from blood to brain were determined in rats by measuring the rate of blood-to-brain glucose transfer at different blood glucose concentrations. Each determination lasted 20 s. For whole-brain, Tmax and Km averaged 258 .+-. 33 (SE) .mu.mol/(100 g) per min and 5.9 .+-. 1.6 (SE) mmol/l, respectively. The regional variations were insignificant. The new approach permits kinetic parameters to be measured locally in brain in rapidly changing functional states.Keywords
This publication has 20 references indexed in Scilit:
- Metabolic response to hypertonic glucose administration in Reye syndromeAnnals of Neurology, 1978
- Enzymatic elimination of substrates flowing through the intact liverJournal of Theoretical Biology, 1976
- OXIDATIVE METABOLISM OF THE CEREBRAL CORTEX OF THE RAT IN SEVERE INSULIN‐INDUCED HYPOGLYCAEMIAJournal of Neurochemistry, 1976
- SOME DYNAMIC ASPECTS OF BRAIN METABOLISM IN RATS GIVEN A PORTOCAVAL ANASTOMOSISNeuropathology and Applied Neurobiology, 1975
- Blood-Brain Transfer of D-Glucose, L-Leucine, and L-Tryptophan in the RatActa Physiologica Scandinavica, 1975
- The transport of glucose into the brain of the rat in vivoProceedings of the Royal Society of London. B. Biological Sciences, 1973
- Reduced brain glucose with normal plasma glucose in salicylate poisoningJournal of Clinical Investigation, 1970
- Notes on the Glucose Oxidase MethodScandinavian Journal of Clinical and Laboratory Investigation, 1967
- The Permeability of Capillaries in Various Organs as Determined by Use of the ‘Indicator Diffusion’ MethodActa Physiologica Scandinavica, 1963
- A Methodological Study of the Enzymatic Determination of Glucose in BloodScandinavian Journal of Clinical and Laboratory Investigation, 1963