SLACS retrotransposon fromTrypanosoma brucei gambienseis similar to mammalian LINEs
Open Access
- 25 February 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (4) , 785-792
- https://doi.org/10.1093/nar/18.4.785
Abstract
We have characterized a retrotransposon in Trypanosoma brucei gambiense uniquely associated with the spliced-leader (SL) RNA gene cluster (Spliced Leader Associated Conserved Sequence, SLACS). There are nine copies of SLACS and DNA sequence analysis of one shows the hallmarks of Line-1 like elements. SLACS has generated a 49 bp target DNA duplication at its insertion site and its 3′-end is preceeded by a poly(A) stretch. Two putative open reading frames (ORFs) span 75% of the element. ORF1 has CysHis motif associated with the retroviral gag polypeptide while ORF2 shows homology with reverse transcriptase sequences. Its 5′-end contains a repeated segment of a 185 bp that varies in copy number in different SLACS insertions. Retrotransposon-like sequences inserted into the SL-RNA genes occur in several hemoflagellates. These elements may represent a related family which has maintained its target site specificity.Keywords
This publication has 31 references indexed in Scilit:
- TRANSCRIPTION AND REVERSE TRANSCRIPTION OF RETROTRANSPOSONSAnnual Review of Microbiology, 1989
- Functional expression of a sequence-specific endonuclease encoded by the retrotransposon R2BmCell, 1988
- jockey, a mobile drosophila element similar to mammalian LINEs, is transcribed from the internal promoter by RNA polymerase IICell, 1988
- DNA-binding fingers encoded by a trypanosome retroposonJournal of Molecular Biology, 1987
- Trypanosoma brucei repeated element with unusual structural and transcriptional propertiesJournal of Molecular Biology, 1987
- The molecular basis of I-R hybrid Dysgenesis in drosophila melanogaster: Identification, cloning, and properties of the I factorCell, 1984
- Complete nucleotide sequence of an unusual mobile element from trypanosoma bruceiCell, 1984
- Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virusNature, 1983
- Ribosomal insertion-like elements in Drosophila melanogaster are interspersed with mobile sequencesCell, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980