Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and β-NGF genes
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (12) , 4761-4770
- https://doi.org/10.1093/nar/15.12.4761
Abstract
A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DBA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for β-Nerve Growth Factor to 2p24 and 1p13 respectively.Keywords
This publication has 21 references indexed in Scilit:
- Proliferation-specific and differentiation-associated chromosomal breakpoints in human neoplasia-a unifying modelHereditas, 2008
- Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridizationHuman Genetics, 1986
- A new hybridocytochemical method based on mercurated nucleic acid probes and sulfhydryl-hapten ligandsHistochemistry and Cell Biology, 1986
- In situ hybridization of an acetylaminofluorene-modified probe recognized by Z-DNA antibodies in vitroCytogenetic and Genome Research, 1986
- Chromosomal localization of a unique gene by non-autoradiographic in situ hybridizationNature, 1985
- Detection of Y-specific repeat sequences in normal and variant human chromosomes using in situ hybridization with biotinylated probesCytogenetic and Genome Research, 1985
- Rapid and high resolution detection of in situ hybridisation to polytene chromosomes using fluorochrome-labeled RNAChromosoma, 1981
- Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry.Journal of Histochemistry & Cytochemistry, 1981
- A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomesCytogenetic and Genome Research, 1978
- Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proceedings of the National Academy of Sciences, 1977