Reversal by Hypotonic Medium of the Antiviral State Induced by Lymphoblastoid Interferon in Human HeLa Cells

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Abstract
The induction kinetics of the antiviral state in HeLa cells treated with human lymphoblastoid interferon (IFN) was studied. In cells treated with 4–200 U/ml IFN, the antiviral state was fully established in 7–9 h. Inhibition of virus multiplication was more rapid if the concentration of IFN was increased to 1,000 U/ml. This antiviral state gradually disappeared during the 48 h after IFN removal. Several compounds known to act on the cell membrane or on the cytoskeleton were tested for their influence on the establishment and reversal of the antiviral state. None of them were found to influence these two parameters to a significant extent. In contrast, placing HeLa cells in medium lacking NaCl partially reversed the blockade to virus multiplication induced by IFN treatment. Cells treated with IFN and later placed in hypotonic medium synthesized virus proteins after encephalomyocarditis virus infection, although at a reduced level compared to cells that had not been treated with IFN.