Calcification of Hypertrophic Epiphyseal Cartilage in vitro Following Inactivation of Phosphatase and Other Enzymes.
- 1 November 1948
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 69 (2) , 262-263
- https://doi.org/10.3181/00379727-69-16684
Abstract
Mineralization of hypertrophic epiphyseal cartilage in vitro was accomplished despite complete and irreversible inactivation of phosphatase and other enzymes preliminary to incubation of the cartilage in the calcifying media. Rachitic cartilage treated with AgCl2 1:1000, HgCl2 10-2 [image], or heated in water to more than 60 C, and as high as 100 C, for 1 hr. retained its capacity to take up Ca phosphate in the same manner as untreated cartilage. Slightly higher critical ion products were required by the treated tissues than by the untreated tissues. Calcification of hypertrophic epiphyseal cartilage in vitro does not require phosphatase or other enzymes.Keywords
This publication has 1 reference indexed in Scilit:
- Calcification of hypertrophic cartilage in vitroBiochemical Journal, 1934