Localization of intrinsic factor and complement fixing intrinsic factor--intrinsic factor antibody complex in parietal cell of man.

  • 1 April 1971
    • journal article
    • Vol. 8  (4) , 517-27
Abstract
In an attempt to localize intrinsic factor in the human parietal cell, and to study its intracellular union with the intrinsic factor antibody and complement, intrinsic factor antibody was separated from coexisting parietal cell antibody in pernicious anaemia sera by gel filtration. Intrinsic factor antibody of both `binding' and `blocking' type was also produced in rabbits by immunization with semi-purified human intrinsic factor–[57Co]B12 complex.