Higher order structure in metaphase chromosomes
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 69 (3) , 363-372
- https://doi.org/10.1007/bf00332139
Abstract
Metaphase chromosomes released from cells in the presence of Joklik's suspension media by vortex-mixing with 0.5 mm glass beads have been analyzed by electron microscopy. In these preparations the chromosomes are composed of series of loops (200–300 Å in diameter) which are, in turn, composed of closely-apposed arrays of nucleosomes. Negative-staining of these preparations has allowed the identification of several distinct patterns within the loop which appear to arise from variations in nucleosome packing. Analogous patterns are also observed in chromatin fragments generated by brief micrococcal nuclease digestion. From these data we have deduced certain features of nucleosome-nucleosome interactions in higher-ordered chromatin fibers.This publication has 25 references indexed in Scilit:
- Structure of nucleosome core particles of chromatinNature, 1977
- The chromosome fiber: Evidence for an ordered superstructure of nucleosomesChromosoma, 1977
- Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogasterCell, 1976
- Solenoidal model for superstructure in chromatin.Proceedings of the National Academy of Sciences, 1976
- Chromatin Structure: Deduced from a MinichromosomeScience, 1975
- The subunit structure of the eukaryotic chromosomeNature, 1975
- Chromatin Structure: A Repeating Unit of Histones and DNAScience, 1974
- Electronmicroscopy of Genetic ActivityAnnual Review of Biochemistry, 1973
- Coiled Supercoiled DNA in Critical Point Dried and Thin Sectioned Human Chromosome FibresNature New Biology, 1971
- Chromosome fibers studied by a spreading techniqueChromosoma, 1966