INHIBITION OF ERYTHROID COLONY FORMATION BY AUTOLOGOUS BONE-MARROW ADHERENT CELLS FROM PATIENTS WITH THE ANEMIA OF CHRONIC DISEASE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 62  (2) , 406-412
Abstract
To determine the role marrow-adherent cells may play in the anemia of chronic diseases, marrow samples were collected from 10 patients with the anemia of chronic disease, 7 control patients with cancer but without the anemia of chronic disease, and 5 normal volunteers. Marrow was either cultured directly or 1st depleted of adherent cells and then cultured. Plasma clots containing 6 .times. 104 nonadherent marrow cells were cocultured with marrow-adherent cells prepared by incubating 6 .times. 104-6 .times. 102 unfractionated marrow cells in microtiter plates and removing the nonadherent cells. Adherent cell depletion of marrow from patients with anemia of chronic disease significantly increased erythroid colony formation. Coculture of adherent cells from anemic patients with autologous nonadherent marrow cells inhibited erythroid colony-forming unit (CFU-E) proliferation in patients with the anemia of chronic disease. In contrast, adherent cells from control patients did not affect autologous erythroid colony formation, and adherent cells from normal volunteers stimulated autologous erythroid colony formation. Coculture of adherent cells from anemia patients with nonadherent marrow from control patients failed to inhibit allogeneic erythroid colony formation. Media conditioned by adherent cells from patients wwith the anemia of chronic sease failed to suppress consistently the formation of allogeneic erythroid colonies. Marrow-adherent cells normally stimulate erythropoiesis, but suppress erythroid progenitors in patients with the anemia of chronic disease and may in part be responsible for their anemia.