Sensitivity of Oral Tissues to Herpes Simplex Virus—in Vitro

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Abstract
Epithelial and fibroblast cells from the human gingival sulcus area were cultivated in vitro, and their sensitivity to the herpes simplex virus (HSV) was studied. Fibroblasts were sensitive to HSV infection and supported virus multiplication as evidenced by nuclear inclusion bodies and a cytopathogenic effect. Epithelial cells which were primarily devoid of HSV antigens were infected with HSV as demonstrated by positive immunofluorescent staining and damage to the cells at a later stage. Epithelial cells that were found to harbor HSV antigens upon removal from the patients prior to infection in vitro, maintained these antigens throughout the period of in vitro cultivation. The sensitivity of both epithelial cells and fibroblasts from the gingival sulcus area to HSV infection in vitro is significant for the understanding of the role of these tissues as a primary site of infection and as a possible reservoir for the latent virus between recurrences.