Effect of ischaemic necrosis and autolysis in vitro on the soluble proteins of rat kidneys

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Abstract
The soluble proteins extracted from perfused kidneys of normal rats were separated by paper electrophoresis into 8 components. The supernatant fluid obtained from unperfused normal kidneys contains, in addition to a hemoglobin band, a component similar in mobility to serum albumin, as well as a faint band probably identical with serum [beta]-globulin. Perfused kidneys transferred to the peritoneal cavities of other rats increase in weight during the first 24 hours, and then decrease in weight rapidly. The total soluble proteins of the kidneys decrease rapidly during the first 4 hours of ischemia and remain practically constant afterwards. In autolysis in vitro, the rapid decrease in soluble proteins lasts for the first 8 hours and is more marked than in the previous case; later, a slower decrease is observed. Paper electrophoresis of the soluble proteins extracted from kidneys left in situ after ligation of the blood vessels, and from kidneys transferred to the peritoneal cavities of other rats, shows a progressive disappearance of the original tissue proteins, accompanied by the appearance of foreign proteins similar to those of the blood serum of the same animals; such proteins were not seen in autolysis in vitro. The interpretation of these findings is discussed.

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